Prevalence of Fasciola spp infestation in slaughtered ruminants using by ELISA, fecal and macroscopic analysis in Ahvaz the capital of Khuzestan Province

Fasciolosis is a zoonotic parasitic disease with worldwide distribution. The disease causes significant economic losses in herd industry including decrease animal products such as meat, milk and infertility reduction. Human can acquire infection as accidental host especially in areas with high level of raining and humidity. Previous studies showed the prevalence of infection among meat-product animals based on inspiration of carcasses in abattoirs using macroscopic test. It seems that the inspiration evidence is not quite precise because the early infection cannot be recognized by macroscopic examination. Therefore, the present cross-sectional study was conducted to determine the Prevalence of fasciolosis on ruminants slaughtered at Ahvaz abattoir, Khuzestan province. Totally, 60 blood and fecal samples of sheep, 50 samples of cattle and 50 samples of buffalo were randomly collected through April to May 2016. Fecal samples were examined using concentration by flotation techniques for the presence of Fasciola ova. The sera from blood sample tested for the presence of anti Fasciola spp antibodies by ELISA technique and livers of slaughter animals were checked for presence of Fasciola spp infestation. In this study, Seroprevalence of fasciolosis was 5% for sheep, 20% for cattle and 18% for buffaloes. The incidence of liver fasciolosis in sheep, cattle and buffaloes samples were 6.6%, 18% and 12% respectively. Microscopic examination in fecal test was negative.